Introduction
The DeNovix DS-11 Spectrophotometer utilizes a proprietary SmartPath™ technology to automatically adjust the microvolume mode pathlengths.
Although routine calibration is not necessary, the following LC-NA reagent may be used with the specified protocol be
Reagents
DeNovix cat #LC-NA is an aqueous nicotinic acid standard used to verify that the microvolume pathlengths are within specifications. SDS and pricing in the US are available at denovix.com/accessories, or contact your distributor for pricing if outside the US.
Protocol
Troubleshooting
Low Absorbance Values
Dirty measurement surfaces or improper blank:
- Clean the surfaces, measure a new Blank using fresh source of dH20 and restart the procedure using a new vial of solution.
High Absorbance Values
Multiple measurements made using the same aliquot:
- Use a fresh aliquot for each measurement.
Pipette tips not changed between aliquots:
- Use a fresh tip to pipette each aliquot onto the sample surface.
The sample surfaces are not adequately cleaned between measurements:
- Between aliquots, wipe both the top and bottom surfaces with a dry lab wipe.
The solution has concentrated due to prolonged exposure:
- Use a fresh vial of solution.
High Variability in Replicates
Multiple measurements made using a single aliquot:
- Use a fresh aliquot for each measurement.
Pipette tips not changed between aliquots:
- Use a fresh tip to pipette each aliquot onto the sample surface.
The sample surfaces are not adequately cleaned between measurements:
- Clean both the top and bottom surfaces between measurements.
Results
Screen captures of the results may be exported via email, saved to a USB device or printed to an approved DeNovix accessory printer.
Summary
If technical support is required, please email a screen capture of the results to techsupport@denovix.com or call us at +1 302.442.6911.
- Please include the serial number of your unit and provide your full contact information (email address and phone number) in the body of the email.
- Please note that responses will not be sent to the email account used by the instrument.
- If contacting us by phone, please have the replicate values handy as we will ask for that information to expedite the troubleshooting process.
Outside of the US, please contact your local distributor for assistance.
18-JAN-2024