This technical note describes how rapid and accurate viable pollen counts can be determined using the DeNovix CellDrop™ FL Automated Cell Counter in conjunction with fluorescein diacetate (FDA) fluorescent stain.
The CellDrop™ FL Automated Cell Counter enables the researcher to count PBMCs using a fluorescence image-based method. This Tech Note describes how CellDrop saves time, money and increases count accuracy and reproducibility vs. manual counting.
Employing best practices is essential for ensuring accurate and representative cell counts. This document explains the key factors that researchers should understand when using the CellDrop Automated Cell Counter.
A detailed description of how to optimize the AOPI or Custom Methods Applications for counting protoplasts on the DeNovix CellDrop™ FL Automated Cell Counter in conjunction with fluorescein diacetate (FDA) and propidium iodide(PI) fluorescent dyes.
Many yeast samples from brewing contain extracellular debris and are small in size, making them difficult to count using a traditional hemocytometer or brightfield cell counter. This technical note will provide the information needed to accurately count yeast concentration and viability using the fluorescent dyes fluorescein diacetate (FDA) and propidium iodide (PI).
The use of trypan blue can be problematic for counting cells and assessing viability in some sample types where debris or non-nucleated cells can interfere. The DeNovix Acridine Orange (Ao) / Propidium Iodide (PI) fluorescence assay is a simple, accurate alternative. Comparative data is presented here.