Tag Archives: Primary Cells

The use of trypan blue can be problematic for counting cells and assessing viability in some sample types where debris or non-nucleated cells can interfere. The DeNovix Acridine Orange (Ao) / Propidium Iodide (PI) fluorescence assay is a simple, accurate alternative. Comparative data is presented here.

The NIST has proposed a robust method to assess cell counting linearity and reproducibility. This technical note presents data for the CellDrop using this method and compares results to manually cell counting.

This technical note outlines seven top tips from the DeNovix applications team to ensure accurate and reproducible results when counting cells manually or using automated systems such as the CellDrop™ Automated Cell Counter. 

EasyApps software includes pre-installed count apps for rapid counting and viability assessment. This note defines the count settings and how each can be adjusted to optimize performance.

The presence of debris, non-nucleated cells and highly variable cell numbers can make counting primary cells challenging. Peripheral Blood Mononuclear Cells (PBMCs) are a common primary cell source where accurate enumeration is essential. This technical note outlines the best practices for counting PBMCs using the CellDrop Automated Cell Counter.