CellDrop Protocol Settings

CellDrop™ Automated Cell Counters include default protocols with count settings that are suitable for many cell types. Optimized settings can improve the accuracy of counts for specific applications and cell lines. See the tables below for suggested protocol parameters.

For additional tips and best practices for successful cell counts, visit the CellDrop Technical Notes page.

Recommended Protocol Settings

SettingsImageSettingsImage
CHO - AO/PI App
Dilution Factor 2
Diameter Minimum 4
Diameter Maximum 23
Live Roundness 1
Dead Roundness 1
Green Threshold 1
Red Threshold 1
CHO - Trypan Blue App
Dilution Factor 2
Diameter Minimum 8
Diameter Maximum 30
Live Roundness 60
Dead Roundness 25
Stained Threshold 35
Small Cell Mode: Off
HEK 293t - AO/PI App
Dilution Factor 2
Diameter Minimum 7
Diameter Maximum 25
Live Roundness 1
Dead Roundness 1
Green Threshold 1
Red Threshold 1
HEK 293t - Trypan Blue App
Dilution Factor 2
Diameter Minimum 8
Diameter Maximum 30
Live Roundness 60
Dead Roundness 13
Stained Threshold 55
Small Cell Mode: Off
Jurkat - AO/PI App
Dilution Factor 2
Diameter Minimum 4
Diameter Maximum 20
Live Roundness 1
Dead Roundness 1
Green Threshold 1
Red Threshold 1
Jurkat - Trypan Blue App
Dilution Factor 2
Diameter Minimum 6
Diameter Maximum 20
Live Roundness 65
Dead Roundness 20
Stained Threshold 30
Small Cell Mode: Off
Mouse PBMC - AO/PI App
Dilution Factor 2
Diameter Minimum 4
Diameter Maximum 20
Live Roundness 1
Dead Roundness 1
Green Threshold 1
Red Threshold 10
Mouse PBMC - Trypan Blue App
Dilution Factor 2
Diameter Minimum 6
Diameter Maximum 20
Live Roundness 35
Dead Roundness 15
Stained Threshold 5
Small Cell Mode: On
Mouse Lymph Node - AO/PI App
Dilution Factor 2
Diameter Minimum 4
Diameter Maximum 20
Live Roundness 1
Dead Roundness 1
Green Threshold 10
Red Threshold 10
Mouse Lymph Node - Trypan Blue App
Dilution Factor 2
Diameter Minimum 6
Diameter Maximum 20
Live Roundness 35
Dead Roundness 15
Stained Threshold 5
Small Cell Mode: On
Whole Blood - AO/PI App
Dilution Factor 200
Min Diameter 4
Max Diameter 15
Live Roundness 1
Dead Roundness 1
Green Threshold 1
Red Threshold 1