Greatest Sensitivity, Widest Dynamic Range
DeNovix Fluorescence Assay Kits provide the greatest sensitivity and widest dynamic range available today. DeNovix offers highly specific assay kits for dsDNA and RNA. DeNovix kits selectively and accurately quantify in the presence of common contaminants such as other nucleic acids or protein. Combine the specificity of fluorescence with the purity measurements of absorbance on our DS-11 FX or DS-11 FX+ for the optimum sample QC procedure.
DeNovix dsDNA Quantification Assays
DeNovix dsDNA Assays cover three overlapping ranges of sensitivity. Each kit includes standards for easy set up of a two-point standard curve. DeNovix Assays have a wider dynamic range than Qubit™ dsDNA assays – and are more affordable. The breakthrough DeNovix dsDNA Ultra High Sensitivity assay allows measurement of samples as low as 0.5 pg/µL (original sample concentration), 20X lower than the core range of the Qubit™ dsDNA HS assay. View our Tech Note comparing dsDNA quantification on DeNovix and Qubit™ Fluorometers.
The range of DeNovix dsDNA assays offered is indicated in the table below.Assay Detection Ranges
|DeNovix dsDNA Assay||Range|
|Broad Range||0.1 – 2000 ng/µL (extended range to 4000 ng/uL)|
|High Sensitivity||10 pg/µL – 250 ng/µL (extended range down to 5 pg/uL)|
|Ultra High Sensitivity||0.5 – 300 pg/µL|
Dynamic Range vs. Qubit™
DeNovix Fluorescence Quantification Assays outperform equivalent Thermo Fisher Qubit™ assay kits in both sensitivity and dynamic range.
DeNovix RNA Quantification Assays
The DeNovix RNA Assay quantifies the widest range of sample concentrations available in a single kit. DeNovix Assays have a wider dynamic range than the Qubit™ Broad Range and High Sensitivity assays combined. The assay measures sample concentrations of 250 pg/µL to 1500 ng/µL RNA. This corresponds to a total mass of 0.5 ng to 1500 ng RNA.
Dynamic Range vs. Qubit™
Performance Results and Comparison
The Figures below demonstrate that the DeNovix RNA Assay enables measurement of RNA through a range of 0.5 ng (20 µL of 0.25 ng/μL) to 1500 ng (1 µL of 1500 ng/µL) by varying mass and volume of samples. The assay performance is linear over the range analyzed with an exceptional R2 value.
DeNovix dsDNA Assays may be used on instruments other than DeNovix Fluorometers. Visit our Qubit™ resources page for downloadable .qbt files which enable Qubit™ fluorometers to quantify dsDNA or RNA with DeNovix assays.
Free Evaluation Kit Request
Please fill out the form below to request a free DeNovix Fluorescence Quantification Assay Evaluation Kit.