AO-PI Viability Assay
The Acridine Orange (AO) / Propidium Iodide (PI) assay is used for cell counting and viability. AO is a nucleic acid-binding fluorophore that is cell membrane permeable and suitable for selective staining of nucleated cells. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells. All live, nucleated cells fluoresce green due to AO, while dead, nucleated cells are stained with both AO and PI and fluoresce red.
Components 1.5 mL @ mix of 5 ug/mL AO+ 100 ug/mL PI in PBS
The Acridine Orange (AO) Dye is used for total cell counting. AO is a nucleic acid-binding fluorophore that is cell membrane permeable and suitable for selective staining of nucleated cells.
Components 1.5 mL @ 10 ug/mL in PBS
The Propidium Iodide (PI) Dye is used for viability assessment. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells.
Components 1.5 mL @ 100 ug/mL in PBS
The Fluorescein Diacetate (FDA) and Propidium Iodide (PI) assay is used for yeast cell viability testing. FDA is a cell viability probe that is membrane-permeable to live yeast cells. Inside a live cell, FDA is cleaved to give fluorescein by the action of intracellular esterases. Propidium Iodide dye for viability assessment. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells. Live cells are reported by FDA in the green channel, dead cells by PI in the red channel.
Components 300 uL PI, 300 uL FDA, 25 mL PBS buffer
The Trypan Blue (TB) dye is used for brightfield viability assessment. TB is an azo dye that is excluded from cells with intact cell membranes, only staining non-viable cells. The trypan blue assay is used to assess the number of viable cells present in a cell suspension.
Components 1.5 mL @ 0.4% in PBS
Count Codes for CellDrop Cell Counters.
The Serial Number is required. Please include when placing your order.
The DeNovix team will process the order and email a count code within one business day. If you have an emergency requirement for additional counts, please call DeNovix at +1-302-442-6911 for immediate help, 9:00 am – 5:00 pm Eastern US time.
Brightfield beads for CellDrop FL and CellDrop BF.
Components 0.5 mL @ 1×106
Fluorescent beads for CellDrop FL.
Components 0.5 mL green / red mix @ 1×106
20 uL Pipette
Capacity 2.0 – 20.0 uL
Performance Verification Slide
The CellDrop PV Slide is used to confirm CellDrop accuracy and precision. The slide contains permanently mounted beads which are analyzed and labeled with a factory certified count and density. The slide can be used for validation of the brightfield, green fluorescence and red fluorescence channels. Factory validation certificate and storage case are included.
Opticon OPI3601 USB Scanner
> Rapidly scans/decodes a wide variety of 1D or 2D barcodes
> Customizable trigger modes
> Distinct targeting guides
> Auto-Trigger features both stand-alone and with stand detection