CellDrop™ Automated Cell Counters include default protocols with count settings that are suitable for many cell types. Optimized settings can improve the accuracy of counts for specific applications and cell lines. See the tables below for suggested protocol parameters.

For additional tips and best practices for successful cell counts, visit the CellDrop Technical Notes page.

 

SettingsImageSettingsImage
CHO - AO/PI App
Dilution Factor 2
Diameter Minimum 4
Diameter Maximum 23
Live Roundness 1
Dead Roundness 1
Green Threshold 1
Red Threshold 1
CHO - Trypan Blue App
Dilution Factor 2
Diameter Minimum 8
Diameter Maximum 30
Live Roundness 60
Dead Roundness 25
Stained Threshold 35
HEK 293t - AO/PI App
Dilution Factor 2
Diameter Minimum 7
Diameter Maximum 25
Live Roundness 1
Dead Roundness 1
Green Threshold 1
Red Threshold 1
HEK 293t - Trypan Blue App
Dilution Factor 2
Diameter Minimum 8
Diameter Maximum 30
Live Roundness 60
Dead Roundness 13
Stained Threshold 55
Jurkat - AO/PI App
Dilution Factor 2
Diameter Minimum 4
Diameter Maximum 20
Live Roundness 1
Dead Roundness 1
Green Threshold 1
Red Threshold 1
Jurkat - Trypan Blue App
Dilution Factor 2
Diameter Minimum 6
Diameter Maximum 20
Live Roundness 65
Dead Roundness 20
Stained Threshold 30
MCF7 - AO/PI App
Dilution Factor 2
Min Diameter 7
Max Diameter 27
Live Roundness 1
Dead Roundness 1
Green Fluorescence Threshold 1
Red Fluorescence Threshold 1
MCF7 - Trypan Blue App
Dilution Factor 2
Min Diameter 6
Max Diameter 40
Live Roundness 50
Dead Roundness 25
Stained Threshold 35
MEF - AO/PI App
Dilution Factor 2
Min Diameter 8
Max Diameter 40
Live Roundness 5
Dead Roundness 5
Green Fluorescence Threshold 10
Red Fluorescence Threshold 20
Irregular Cell Mode: On
MEF - Trypan Blue App
Dilution Factor 2
Min Diameter 6
Max Diameter 40
Live Roundness 50
Dead Roundness 25
Stained Threshold 35
Irregular Cell Mode: On
Mouse PBMC - AO/PI App
Dilution Factor 2
Diameter Minimum 4
Diameter Maximum 20
Live Roundness 1
Dead Roundness 1
Green Threshold 1
Red Threshold 10
Mouse PBMC - Trypan Blue App
Dilution Factor 2
Diameter Minimum 6
Diameter Maximum 20
Live Roundness 35
Dead Roundness 15
Stained Threshold 5
Small Cell Mode: On
Mouse Lymph Node - AO/PI App
Dilution Factor 2
Diameter Minimum 4
Diameter Maximum 20
Live Roundness 1
Dead Roundness 1
Green Threshold 10
Red Threshold 10
Mouse Lymph Node - Trypan Blue App
Dilution Factor 2
Diameter Minimum 6
Diameter Maximum 20
Live Roundness 35
Dead Roundness 15
Stained Threshold 5
Small Cell Mode: On
MRC5 - AO/PI App
Dilution Factor 2
Min Diameter 4
Max Diameter 45
Live Roundness 1
Dead Roundness 1
Green Fluorescence Threshold 1
Red Fluorescence Threshold 1
Irregular Cell Mode: On
MRC5 - Trypan Blue App
Dilution Factor 2
Min Diameter 4
Max Diameter 40
Live Roundness 50
Dead Roundness 35
Stained Threshold 35
Irregular Cell Mode: On
Whole Blood - AO/PI App
Dilution Factor 200
Min Diameter 4
Max Diameter 15
Live Roundness 1
Dead Roundness 1
Green Threshold 1
Red Threshold 1