Whether you're curious about optimizing sample prep or LED selection, check out this resource on the best practices for fluorescence measurement.
QFX Fluorometer
Powerful Quantification
The QFX Fluorometer is more sensitive and reproducible than any other instrument in its class. The QFX is a powerful solution for all fluorometric applications where enhanced specificity or sensitivity are needed. Quantify nucleic acids and proteins using four user-selectable fluorescent channels with a choice of common assays or easily create custom apps for the ultimate in fluorometer flexibility. The QFX outperforms Qubit™ and other tube fluorometers giving superior data quality, more powerful software and full network integration for seamless data handling.
Combined with the DeNovix Fluorescence Assays, the QFX offers quantification of DNA between 0.5 pg/µL and 4000 ng/µL. The ideal solution for quantification of degraded or low concentration samples, even in the presence of common contaminants.
Easy to Learn. Fast to Use.
The QFX features the most powerful and intuitive software in its class. Pre-installed DeNovix EasyApps™ and an intuitive touchscreen interface ensure error-free operation and sample to data in under 2 seconds. Every instrument is ready right out of the box – no PC set-up or software installation required.
- Autosave up to 2 million samples.
- Four user-selectable fluorescent channels
- Compatible with all common quantification assays
- Easily create custom assays
Four User-Selectable Fluorescent Channels for Greater Assay Choice
DeNovix Fluorometers include four user-selectable emission and excitation channels to provide scientists the flexibility to use the fluorophore and detection ranges of your choice. Choose from UV (375 nm), Blue (470 nm), Green (525 nm) or Red (635 nm) excitation sources.
Fluorescence Freedom!
DeNovix fluorometers give scientists the flexibility to choose any fluorescent assay, not just the assays supplied by one manufacturer. Fluorometer apps are compatible with DeNovix Fluorescence Assays and other common commercially available assays measured in standard 0.5 mL thin-wall PCR tubes. Assays include:
dsDNA: DeNovix dsDNA Broad Range, DeNovix dsDNA High Sensitivity, DeNovix dsDNA Ultra High Sensitivity, Quant-iT PicoGreen™, Quant-iT dsDNA HS, Quant-iT dsDNA BR, Qubit™ dsDNA BR, Qubit™ dsDNA HS, Quantifluor™ dsDNA and Quantifluor™ One dsDNA
RNA: DeNovix RNA Assay, Quant-iT Ribogreen, Quant-iT RNA BR, Quant-iT RNA HS, Qubit™ RNA BR, Qubit™ RNA HS, Quantifluor RNA, Quant-iT microRNA, Qubit™ MicroRNA
ssDNA: Quant-iT OliGreen, Qubit™ ssDNA, Quantifluor ssDNA
Protein: Quant-iT Protein, Qubit™ Protein
Unrivaled Connectivity
Unlike any other instrument in this class, the QFX is fully network integrated. Built-in Wi-Fi, Ethernet and USB allow easy for export of the data you want in the format you need. The QFX also has onboard storage capacity for over 2 million samples.
- Email a csv or color jpg of your results right from EasyApps
- Automatically save data to network drives or LIMS
- Print results or graphs to network and label printers
- Save to USB
21 CFR Part 11 Compliance Ready
EasyApps Secure provides an optional suite of software controls that allow regulated GxP facilities to easily add DS-11 Series instruments to their compliant workflows (view the compliance statement). The software is fully integrated within the on-board operating system and includes a range of features essential to ensuring compliance, including:
- Password protected system access
- Integrated electronic signature controls
- Comprehensive user account management
- Secure audit trail recording
- Advanced data handling and export tools
Software Features
Nucleic Acid Quantification
- Compatible with all commonly used quantification assays including Qubit™, Quant-it™ and Quantifluor™
- Easy configuration of additional assays
- Single or multi-replicate standard curve options
- User control of line fit, trend line analysis, subtraction of blank
- User control of assay and reported sample concentration units
- Save and re-use curves and lists of standard concentrations
Custom Fluorescence Apps
- Add new standard curve based fluorescent assays (nucleic assay, protein or other)
- Basic Fluorometer app returns RFU values for a wide range of fluorophores with four user-selectable fluorescent channels
Key Features
- Compatible with all commonly used protein quantification assays
- Easy configuration of additional assays
- Single or multi-replicate standard curve options
- User control of line fit, trend line analysis, subtraction of blank
- User control of assay and reported sample concentration units
- Save and re-use curves and lists of standard concentrations
User Accounts and Data Handling
- Password protected accounts available
- All data automatically saved & searchable by account, sample name & type, application and date
- Configurable reports easily accommodate LIMS and user preferred formats
- Print microfuge tube labels using a DeNovix supported printer
- Print reports to a networked printer or DeNovix supported label printer
- Export full data sets via email; save to a network folder or USB thumb drive.
Exporting
Simply and securely connect the DS-11 to your network by Wifi or Ethernet to enable data exporting by email, or print reports to network or label printers. Each measurement is automatically saved to a large onboard memory enabling storage and retrieval of millions of samples. For labs requiring external data storage, network folder locations can be defined for each user account to automatically export and archive results.
Specifications
Fluorescence Detection
| Light Sources | Blue LED (~470 nm), Green LED (~525 nm), Red LED (~635 nm), UV LED (~375 nm) |
| Excitation Filters | Blue: 442-497 nm, Green: 490-558 nm, Red: 613-662 nm, UV: 361-389 nm |
| Emission Filters | Blue: 514-567 nm, Green: 565-650 nm, Red: 665-740 nm, UV: 435-485 nm |
| Detectors | Photodiode, detection range 300-1000 nm |
| Tube Type | 0.5 mL Real Time thin-wall PCR tube (polypropylene) |
Onboard Controller (No PC required)
| Operating System | Custom Linux® OS |
| CPU | Quad-core Cortex-A72 |
| Display | 7″ high definition color display |
| User Input | Touch screen with swipe and pinch motion; glove compatible |
| Internal Storage | 32GB flash drive |
| Connectivity | 2 USB ports for printer, barcode reader and USB drive |
| Networking | Ethernet and Wi-Fi |
General
| Colors | Arctic White |
| Measurement Time | 2 seconds |
| Footprint | 20 cm x 27 cm |
| Weight | 1.1 kg |
| Operating Voltage | 12 VDC |
| Operating Power Consumption | 10 W; max 30 W |
| Approvals | CE, UL/CSA, FCC and Japan CAB |
| Manufacture Location | USA |
Absorbance and fluorescence are unique but complementary methods for assessing concentration and sample QC. Download this free infographic.
In this eBook, you’ll find resources that discuss the advantages of each method and when to employ Absorbance, Fluorescence or both.
Quantus™ and QuantiFluor™ are registered trademarks of Promega Corporation and its subsidiaries and are used for identification and references purposes only. DeNovix, DeNovix products and this website are not endorsed or authorized by or in any way affiliated with Promega Corporation.
Qubit™, Quant-iT and PicoGreen™ are registered trademarks of Thermo Fisher Scientific and its subsidiaries and are used for identification and references purposes only. DeNovix, DeNovix products and this website are not endorsed or authorized by or in any way affiliated with Thermo Fisher Scientific.
