Isolating nuclei is a critical step for single cell RNA-sequencing and ATAC-sequencing workflows. Data is presented demonstrating the advantages of using the CellDrop Automated Cell Counted for accurately counting isolated nuclei, residual intact cells and debris.
The DeNovix® dsDNA Ultra High Sensitivity Assay enables the accurate detection of purified double-stranded DNA (dsDNA) samples with a standard detection range of 0.5 pg – 300 pg total mass in 200 µL volumes and is compatible with a range of fluorometers and microplate readers.
Fluorescein and its derivative fluorescein isothiocyanate (FITC) are ubiquitous in cell and molecular biology as a fluorescent label for cells and in many other applications. The DeNovix® DS-11 Series Spectrophotometer / Fluorometer instruments enable precise fluorescein quantification using a proprietary optical core and four versatile fluorescence channels.
A short video outlining the best practices for loading and cleaning using DirectPipette, the unique sample retention and measurement technology of CellDrop Automated Cell Counters