The DeNovix quantification assays offer the greatest dynamic range and sensitivity available for fluorescence measurements of dsDNA. While all assays are pre-programmed into the DS-11 FX and QFX instruments, users of Qubit® fluorometers can also take advantage of the improved performance of DeNovix assays. Below are protocols and downloadable assay files (.qbt) for Qubit 2.0 and Qubit 3.0/Qubit 4 fluorometers. Note that due to limitations of their optical systems, Qubit instruments cannot be used for DeNovix Broad Range assays.
Limitations in sensitivity are outlined in the table below.
Dynamic Range of DeNovix dsDNA assays on DS-11 Series vs. Qubit Fluorometers
Protocols
160 DeNovix High Sensitivity Assay Protocol for Qubit fluorometers
161 DeNovix Ultra High Sensitivity Assay Protocol for Qubit 2 fluorometers
207 DeNovix High Sensitivity Assay Protocol for Qubit 3.0 and Qubit 4 fluorometers
208 DeNovix Ultra High Sensitivity Assay Protocol for Qubit 3.0 and Qubit 4 fluorometers
Download Files
DeNovix RNA Assay .qbt file Qubit 2.0
DeNovix RNA Assay .qbt file Qubit 3.0 and Qubit 4
DeNovix High Sensitivity Assay .qbt file Qubit 2.0
DeNovix High Sensitivity Assay .qbt file Qubit 3.0 and Qubit 4
DeNovix Ultra High Sensitivity Assay .qbt file Qubit 2.0
DeNovix Ultra High Sensitivity Assay .qbt file Qubit 3.0 and Qubit 4