Fluorescence Quantification Assays

DeNovix Fluorescence Assay Kits provide the greatest sensitivity and widest dynamic range available today. DeNovix offers  highly specific assay kits for dsDNA and RNA. DeNovix kits selectively and accurately quantify in the presence of common contaminants such as other nucleic acids or protein. Combine the specificity of fluorescence with the purity measurements of absorbance on our DS-11 FX or DS-11 FX+ for the optimum sample QC procedure.

DeNovix Assay Kit transp

 

 ✔   Widest Dynamic Range

dsDNA 0.5 pg/µL – 4000 ng/µL
RNA 250 pg/µL – 1000 ng/µL

 ✔   dsDNA 20X Greater Sensitivity than Qubit®

 ✔   One RNA kit replaces full Qubit® Range

 ✔   Integrated into EasyApps® Software

 ✔   Simple Mix-and-Measure Assay 

 


DeNovix dsDNA Assays

 

DeNovix dsDNA Assays cover three overlapping ranges of sensitivity. Each kit includes standards for easy set up of a two-point standard curve. DeNovix Assays have a wider dynamic range than Qubit® dsDNA assays – and are more affordable. Our breakthrough dsDNA Ultra High Sensitivity assay allows measurement of samples as low as 0.5 pg/µL (original sample concentration), 20X lower than the core range of the Qubit® dsDNA HS assay.


Dynamic Range

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DeNovix dsDNA Assays may be used on instruments other than the DeNovix DS-11 Series of fluorometers. Use the Detailed Protocol links below for additional information. Click here for downloadable Qubit Fluorometer .qbt files for use on Qubit® 2.0 and 3.0 Fluorometers.

Microplates
For those labs requiring higher throughput, all DeNovix assays are also compatible with microplate formats.


DeNovix RNA Assays

 

The DeNovix RNA Assay quantifies the widest range of sample concentrations available in a single kit. DeNovix Assays have a wider dynamic range than the Qubit® Broad Range and High Sensitivity assays combined. The assay measures sample concentrations of 250 pg/µL to 1500 ng/µL RNA.  This corresponds to a total mass of 0.5 ng to 1500 ng RNA.

Performance Results

The Figures below demonstrate that the DeNovix RNA Assay enables measurement of RNA through a range of 0.5 ng (20 µL of 0.25 ng/μL) to 1500 ng (1 µL of 1500 ng/µL) by varying mass and volume of samples. The assay performance is linear over the range analyzed with an exceptional R2 value.

For Research Use Only
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