During our Automated Organoid Counting webinar, we conducted a live Q&A session with our applications team. Learn more.
Optimized for CellDrop™ Cell Counting Workflows
DeNovix CellDrop Assay Kits are simple to use and designed for Brightfield and Fluorescence applications preloaded on CellDrop instruments. DeNovix offers multiple assay kits for cell counting viability assessment, including Acridine Orange / Propidium Iodide (AO/PI) for the CellDrop Fluorescence Cell Counters.
AO/PI Viability Assay
The Acridine Orange (AO) / Propidium Iodide (PI) assay is used for cell counting and viability. AO is a nucleic acid-binding fluorophore that is cell membrane permeable and suitable for selective staining of nucleated cells. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells. All live, nucleated cells fluoresce green due to AO, while dead, nucleated cells are stained with both AO and PI and fluoresce red.
Acridine Orange
The Acridine Orange (AO) Dye is used for total cell counting. AO is a nucleic acid-binding fluorophore that is cell membrane permeable and suitable for selective staining of nucleated cells.
Propidium Iodide
The Propidium Iodide (PI) Dye is used for viability assessment. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells.
Yeast
The Fluorescein Diacetate (FDA) and Propidium Iodide (PI) assay is used for yeast cell viability testing. FDA is a cell viability probe that is membrane-permeable to live yeast cells. Inside a live cell, FDA is cleaved to give fluorescein by the action of intracellular esterases. Propidium Iodide dye is used for viability assessment. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells. Live cells are reported by FDA in the green channel, dead cells by PI in the red channel.
Trypan Blue
The Trypan Blue (TB) dye is used for brightfield viability assessment. TB is an azo dye that is excluded from cells with intact cell membranes, only staining non-viable cells. The trypan blue assay is used to assess the number of viable cells present in a cell suspension.
Erythrosin B
Erythrosin B is a biosafe and non-toxic colorimetric dye that can be used as an alternative to Trypan Blue for cell counting and viability assessment. Evaluation of cell viability using Erythrosin B dye exclusion is based on a similar principle to Trypan Blue, that intact cell membranes exclude the polar molecules whereas compromised membranes do not. Live cells remain unstained while dead cells are stained and have a darker appearance.
Apoptosis
The CellDrop Apoptosis Assay (100 assays kit) leverages the CellDrop FLxi’s 10x objective and dual fluorescence capabilities. Using Annexin V and Propidium Iodide, the kit accurately stains cells to differentiate between live, early apoptotic, late apoptotic, and necrotic populations. Researchers gain comprehensive data on cell death mechanisms and cellular health with unparalleled ease and speed, making critical cell biology studies more efficient.
CHO 293 Cells with Trypan Blue Viability Assay
Mouse Primary Cells with AO/PI Viability Assay
For Research Use Only
Technical Note 244 outlines the apoptosis assay protocol, which is used in conjunction with the CellDrop Automated Cell Counter\'s Apoptosis app.
The CellDrop FLxi offers an efficient and reliable solution for yeast health monitoring, enabling brewers to optimize pitching rates, monitor fermentation rates, and reduce batch variability.
The CellDrop Automated Cell Counter offers a simple, reliable solution for automated organoid counts. Learn more in this webinar.
In this video, learn how the CellDrop Automated Cell Counter's flexible settings can be optimized for your sample type.
CellDrop's convenient dilution calculator automatically calculates target dilution volumes of cell culture and buffer based on each cell count.
