Isolating nuclei is a critical step for single nuclei sequencing and ATAC-workflows. Dual fluorescence analysis using the CellDrop automated cell counter can clearly distinguish nuclei, unlysed intact cells and debris.
Isolating nuclei is a critical step for single cell RNA-sequencing and ATAC-sequencing workflows. Data is presented demonstrating the advantages of using the CellDrop Automated Cell Counted for accurately counting isolated nuclei, residual intact cells and debris.
The DeNovix® dsDNA Ultra High Sensitivity Assay enables the accurate detection of purified double-stranded DNA (dsDNA) samples with a standard detection range of 0.5 pg – 300 pg total mass in 200 µL volumes and is compatible with a range of fluorometers and microplate readers.
Understanding best practice for preparing and measuring your samples when using microvolume spectrophotometers will avoid sources of error that negatively affect the accuracy of results and impact the downstream analysis of a sample
Fluorescein and its derivative fluorescein isothiocyanate (FITC) are ubiquitous in cell and molecular biology as a fluorescent label for cells and in many other applications. The DeNovix® DS-11 Series Spectrophotometer / Fluorometer instruments enable precise fluorescein quantification using a proprietary optical core and four versatile fluorescence channels.
The DeNovix® AO/PI Assay and the AO/PI app on CellDrop™ Series instruments enable rapid automated cell counting for live/dead cell suspensions.