Methods for Improved Cell Counting Accuracy

Trypan Blue is widely used in cell culture labs for selectively staining dead cells and tissues. However, some sample types can present challenges for accurately assessing cell counts and viability with Trypan Blue.

For these sample types, a fluorescence assay such as Acridine Orange / Propidium Iodide (AO/PI) is a simple and highly accurate alternative.

In this eBook, you’ll learn about each viability method and the situations where fluorescence is advantageous.