Automated Cell Counter Principles

Automated cell counters offer a convenient solution to the challenges facing life science researchers today by providing precise results in a fraction of the time taken for manual cell counting. CellDrop™ systems from DeNovix build on the fundamental throughput benefits of established automated cell counting principles with a suite of additional usability advantages.

By eliminating the use of disposable plastic slides and streamlining the entire workflow, the CellDrop Automated Cell Counter also provides a range of cost improvements over conventional cell counting and viability tests.

In this blog post, DeNovix will explore some of the principles of automated cell counters in more detail.

Key Advantages of Automated Cell Counters: Overview

  • Automate cell counting and viability testing with accuracy and reproducibility
  • Eliminate the subjectivity of manual cell counting
  • Create convenient user-defined protocols to standardize between users and labs.
  • Image live and dead cells automatically with image-based processing
  • Improve profitability by reducing operating times and eliminating slide costs
  • Expand the number of available applications
  • Reduce plastic waste in your facility

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How Do CellDrop Automated Cell Counters Work?

CellDrop Automated Cell Counters systematize the basic principles of manual cell counting. Samples are assayed with an appropriate staining reagent and a volume is pipetted into a sample chamber formed between two optical-grade sapphire surfaces. Maintaining precise control of sample chamber height and field of view enables the calculation of the exact volume of cells counted, delivering accurate cell enumeration and viability assessment. Cells are simply wiped from the surface with a dry laboratory wipe ready for the next sample.

The CellDrop Series of automated cell counters uses a novel combination of both fluorescence and brightfield optics, with a series of dedicated applications providing default protocols for various assays and standardized processes. With a mechanically-variable height chamber, the CellDrop offers unprecedented accuracy over the widest range of cell densities (– 7 x 102 – 2.5 x 107) and cell diameters (4 – 400 µm). This provides the basis for the most flexible range of cell counting applications for a single instrument on the market.

For more information on how to get the most out of your instrument, refer to our previous article Best Practices for CellDrop Series Cell Counters

CellDrop Automated Cell Counters: Specifications & Applications

  • Measurement Speed:
  • Brightfield = 3 seconds
  • Fluorescence = 8.5 seconds
  • Measuring Range: 4 – 400 µm
  • Sample Volumes:
  • 0 µL (high density)
  • 10 µL (standard)
  • 40 µL (low density)
  • Dynamic Range: – 7 x 102– 2.5 x 107
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With unmatched flexibility and powerful data reporting, the CellDrop range of automated cell counters can perform a comprehensive range of counting and viability procedures using a host of proprietary and standardized assays. For more insight into using the CellDrop cell counter for specific applications, read our recent blog post Automated Cell Counters: Optimizing for GFP Applications.

Automated Cell Counters from DeNovix

DeNovix is one of the industry-leading suppliers of high analytical instrumentation designed with day-to-day usability in mind. If you would like to talk about quotes or pay-as-you-go contracts for a CellDrop automated cell counter, simply contact a member of the DeNovix team today.