Kit Contents
Kits contain 0.4% Trypan Blue in PBS. The Trypan Blue reagent should be stored at room temperature (15 – 30°C) in an airtight container and does not need to be protected from light.
Assay Size | Trypan Original Concentration | Number of Tests |
---|---|---|
0.25 mL | 0.4% | 50 |
0.2% | 100 | |
1.5 mL | 0.4% | 300 |
0.2% | 600 |
Sample Prep
- Mix cell suspension and Trypan Blue immediately prior to use.
- Optional: Filter Trypan solution through a 0.2 µm filter to remove aggregates and crystals that can form in Trypan solution over time.
- For each sample, mix Trypan and a cell suspension together at the desired ratio and vortex. Refer to the table below for Dilution Factor (DF) guidance examples.
Trypan Volume | Cell Volume | Protocol Dilution Factor | Recommended Exposure |
---|---|---|---|
5 µL 0.4% | 5 µL | 2 | Normal |
2.5 µL 0.4% | 7.5 µL | 1.33 | Low |
Best Practices
Sample Measurement
- Ensure that the arm is in the down position and launch the Trypan Blue app.
- Clean the sample surfaces if there is visible debris in the preview image.
- Optional: Enter a sample name and any additional sample information.
- Select or create a protocol, and set the Dilution Factor according to the table above.
- With the arm down, dispense the sample aliquot into the measurement chamber using the groove on the lower sample surface as a pipetting guide.
- Note: The volume of sample required depends on the protocol settings for the chamber height. The required volume is displayed on the Count button.
- Adjust the focus and exposure.
- When the cells have settled and are no longer moving, tap the Count button.
Refer to Technical Note 186 – CellDrop Best Practices for additional guidance.
Refer to denovix.com/sds for safety data sheets for CellDrop Cell Counting Assays.
01-MAR-2023