Comparing Fluorescence and Trypan Blue Automated Cell Counting

Trypan Blue is widely used in cell culture labs for selectively staining dead cells and tissues. The use of Trypan Blue dye can present challenges for accurately assessing cell counts and viability in some sample types. For these samples, a fluorescence assay such as Acridine Orange / Propidium Iodide (AO/PI) is a simple and highly accurate alternative.

Using the CellDrop Automated Cell Counter, DeNovix Scientific Director Dan Schieffer demonstrates both brightfield and fluorescence methods of cell counting and viability assessment.