DeNovix Instrumentation, Technology and Developments

28 Nov

Automated Cell Counters: Optimizing for GFP Applications

With three distinct optical channels (dual fluorescence and brightfield illumination), CellDropTM Automated Cell Counters offer the utmost in flexibility to satisfy the demands of advanced cell culture laboratories. CellDrop is the most powerful cell counter platform on the market with the widest current feature set for myriad application requirements. These features are typified by the

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06 Nov

CellDrop: Tips for Cell Counting Accuracy

Conventional cell counting techniques are limited by human error and subjectivity, which makes it difficult to achieve a true measure of viability using a hemocytometer and manual microscope. Automated systems surpass manual cell counters in terms of both throughput and precision, yet it is important to follow a simple set of best practices to guarantee

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26 Sep

Beads for Cost-Effective Cell Counter Training

CellDropTM Automated Cell Counters use key principles of Industry 3.0 (automation, combined accuracy and precision, ease-of-use, etc.) to simplify the daily counting and viability tests conducted by cell biologist. As with any new technology, however, implementation, training and validation times may be factors of concern to those familiar with established cell counting technologies. At the

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20 Aug

Peripheral Blood Mononuclear Cell Counting

Quantifying and qualifying the viability of primary cells can be difficult via conventional cell counting methodologies (hemocytometry, slide-based microscopy, etc.). Primary cells are cells that have been harvested directly from living tissues and isolated in vitro. They are not modified like artificially preserved cell lines, which makes them a better representation of the cells’ in

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30 Jul

Operating Cell Counters with FDA/PI Yeast Assays

Viability is a key metric in determining the activity or metabolism of yeast cells in solution. It is commonly measured using either brightfield microscopy or fluorescent stains like fluorescein diacetate (FDA) and propidium iodide (PI) with both manual and automated cell counters. Fluorescein diacetate is an esterase substrate that can freely permeate the plasma membrane

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18 Jul

Manual vs. Automated Cell Counters

Cell counting is a procedure with a choice of numerous diverse techniques, most of which rely on specialized laboratory appliances. Despite the variety of cell counters available for modern-day life science applications, they can typically be divided into one of two sub-groups: manual and automated cell counters. In this blog post, DeNovix compares the benefits

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10 Jun

NanoDropTM One vs DS-11 Series: Comparing Microvolume Performance

Ultraviolet-visible (UV-Vis) spectrophotometry is one of the leading techniques in nucleic acid and protein quantification due to its wide variety of sample measurement capabilities and broad, dynamic range. UV-Vis is a key tool for forensic scientists, microbiologists, and life scientists. This innovative technology has been implemented for genomics and proteomics research, cell biology studies, bioengineering

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03 Jun

Outlining UV-Vis DNA Quantification

There are numerous methods suitable for quantifying the number of nucleic acids in a sample, but UV-Vis absorbance is the primary method for determining a sample’s concentration and purity of DNA or RNA. Both single- and double-stranded DNA strongly absorb ultraviolet light with a peak absorbance wavelength of 260nm. Simple concentration and purity measurements involve

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29 May

Cell Counting with Acridine Orange and Propidium Iodide (AO/PI)

Various methods of cell counting exist for quantifying the total number of cells in solution and calculating sample viability. The conventional method involves loading a sample into a cumbersome hemocytometer and observing particle concentration. A grid etched on the surface of the slide is used to measure cell density and determine the total cell count

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01 May

Comparing DS-11 Series and NanoDrop™ Protein Concentration Capabilities

Microvolume absorbance spectrophotometers are ubiquitous in laboratories and analytical facilities concerned with protein analysis, extraction, and purification. The two leading technologies for protein concentration measurements in biochemistry and life science applications are the DS-11 Series combined spectrophotometer / fluorometer from DeNovix, and the NanoDropTM family of instruments from Thermo Fisher Scientific. Each of these analytical

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