DeNovix Instrumentation, Technology and Developments

20 Aug

Peripheral Blood Mononuclear Cell Counting

Quantifying and qualifying the viability of primary cells can be difficult via conventional cell counting methodologies (hemocytometry, slide-based microscopy, etc.). Primary cells are cells that have been harvested directly from living tissues and isolated in vitro. They are not modified like artificially preserved cell lines, which makes them a better representation of the cells’ in

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30 Jul

Operating Cell Counters with FDA/PI Yeast Assays

Viability is a key metric in determining the activity or metabolism of yeast cells in solution. It is commonly measured using either brightfield microscopy or fluorescent stains like fluorescein diacetate (FDA) and propidium iodide (PI) with both manual and automated cell counters. Fluorescein diacetate is an esterase substrate that can freely permeate the plasma membrane

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18 Jul

Manual vs. Automated Cell Counters

Cell counting is a procedure with a choice of numerous diverse techniques, most of which rely on specialized laboratory appliances. Despite the variety of cell counters available for modern-day life science applications, they can typically be divided into one of two sub-groups: manual and automated cell counters. In this blog post, DeNovix compares the benefits

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10 Jun

NanoDropTM One vs DS-11 Series: Comparing Microvolume Performance

Ultraviolet-visible (UV-Vis) spectrophotometry is one of the leading techniques in nucleic acid and protein quantification due to its wide variety of sample measurement capabilities and broad, dynamic range. UV-Vis is a key tool for forensic scientists, microbiologists, and life scientists. This innovative technology has been implemented for genomics and proteomics research, cell biology studies, bioengineering

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03 Jun

Outlining UV-Vis DNA Quantification

There are numerous methods suitable for quantifying the number of nucleic acids in a sample, but UV-Vis absorbance is the primary method for determining a sample’s concentration and purity of DNA or RNA. Both single- and double-stranded DNA strongly absorb ultraviolet light with a peak absorbance wavelength of 260nm. Simple concentration and purity measurements involve

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29 May

Cell Counting with Acridine Orange and Propidium Iodide (AO/PI)

Various methods of cell counting exist for quantifying the total number of cells in solution and calculating sample viability. The conventional method involves loading a sample into a cumbersome hemocytometer and observing particle concentration. A grid etched on the surface of the slide is used to measure cell density and determine the total cell count

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01 May

Comparing DS-11 Series and NanoDrop™ Protein Concentration Capabilities

Microvolume absorbance spectrophotometers are ubiquitous in laboratories and analytical facilities concerned with protein analysis, extraction, and purification. The two leading technologies for protein concentration measurements in biochemistry and life science applications are the DS-11 Series combined spectrophotometer / fluorometer from DeNovix, and the NanoDropTM family of instruments from Thermo Fisher Scientific. Each of these analytical

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20 Dec

Fluorescence Assays and the Benefits of Multiple Fluorescence Channels

Fluorescence assays are critical in biochemical and life sciences applications using fluorometry for sample quantification. These comprise fluorophores that bind specifically to analytes of interest and exhibit distinct excitation and emission profiles. In principle, the intensity of light emitted is directly correlative to sample concentration. Leading-edge fluorescence assays enable extremely precise biomolecule detection with, for

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26 Nov

Fluorescence Analysis: 4 Differences Between Absorbance and Fluorescence

Absorbance and fluorescence analysis are distinct yet complementary techniques used to detect and quantify analytes of interest in a sample. Measuring the absorbance of ultraviolet and visible (UV-Vis) light at specific wavelengths is one of the most established methods of quantitating biomolecules. Fluorescence analysis, meanwhile, has long been used to measure the emission spectra of

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02 Nov

Microvolume Analysis of Protein Samples

Measuring the UV-light absorption of proteins is the most straightforward method of microvolume protein analysis for concentration and purity. The aromatic amino acids and residues of many proteins exhibit strong ultraviolet absorption with a peak at 280nm.  The amount of light absorbed is proportional to the concentration of the sample. Using the Beer-Lambert equation, it

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