SmartPath Technology enables the DS-11 Series to measure ultra-high concentrations. In this technical note, the relationship between transmittance and absorbance are discussed in the context of the Beer-Lambert Law to explain how the industry-leading dynamic range of the DS-11 is achieved.
Learn about ultraviolet-visible (UV-Vis) spectrophotometry, a technique used to measure light absorbance across the ultraviolet and visible ranges of the electromagnetic spectrum.
The choice of measurement method for proteins depends on sample type, buffers used and the protein of interest. Colorimetric assays are commonly used for cell lysates and samples in buffers that interfere with direct quantification at 280nm. Common assays and results using microvolume analysis are presented in technical note 108.