CellGlow Viability Assay Protocol
The CellGlow AO/PI Viability Assay streamlines cell counting by replacing liquid reagents with precision-coated, surface-stabilized fluorophores in a strip-tube format.
CellGlow is compatible with CellDrop FLi, CellDrop FLxi and slide-based fluorescence slide counters equipped with excitation and emission hardware optimized for Acridine Orange and Propidium Iodide.
- Ensure that the cell suspension is well-mixed and aspirate 20 µL.
- Pierce the seal of CellGlow well using the pipette tip and dispense the sample to the bottom of the tube.
- Note: Ensure that the sample is fully collected at the bottom of the well.
- Mix the solution 2 – 3 times. No incubation time is required.
- Immediately after mixing, aspirate 10 µL of cell suspension from the well and load into the CellDrop Automated Cell Counter chamber or slide.
- Note: A dilution factor of 1 should be used for un-diluted samples when using CellGlow.
- When using liquid-based Viability Assays the dilution factor default is 2 as assay preparation uses a 1:1 volume of dye and cell suspension. There is no dilution effect with CellGlow.
- Each tube strip can be torn away for disposal after use.
- Storage and Shelf-Life:
- Keep at room temperature
- Replace after 12 months
Request Support or Reorder
If you need additional support using the CellGlow Viability Assay, contact our application team using the button below. Select “Reorder CellGlow” if you’d like to purchase more. For international customers, please contact your local distributor.
17-JUL-2026
