CellGlow Viability Assay Protocol
The CellGlow AO/PI Viability Assay streamlines cell counting by replacing liquid reagents with precision-coated, surface-stabilized fluorophores in a 96 well format.
Review this protocol in full before using CellGlow with the CellDrop Automated Cell Counter or any slide-based cell counter.
- Ensure that cells are well-mixed, then aspirate 20 µL of cell suspension.
- In a single motion, pierce one of CellGlow’s individually sealed wells using the pipette tip, then dispense the sample to the bottom of the assay well.
- Note: Ensure that the sample is fully collected at the bottom of the well.
- Mix the solution 2 – 3 times. No incubation time is necessary.
- Immediately after mixing, aspirate 10 µL of cell suspension from the well and load onto an automated cell counter.
- Note: A dilution factor of 1 should be used for un-diluted samples. When using liquid DeNovix CellDrop Viability Assays, dyes will dilute cells 50/50. The DeNovix CellDrop Automated Cell Counter dilution factor default is 2, and most other cell counters include the same standard. Ensure that the cell counter dilution factor is set to 1 when measuring un-diluted samples with CellGlow.
- When all wells have been used within a column, each tube strip can be torn away for disposal. Remove CellGlow from the tray to easily remove a strip.
Request Support or Reorder
If you need additional support using the CellGlow Viability Assay, contact our application team using the button below. Select “Reorder CellGlow” if you’d like to purchase more. For international customers, please contact your local distributor.
