Fluorometric sample quantification is a routine application in molecular biology labs however it is important to employ a number of best practices to ensure reliable results. This document outlines some of the key considerations.
Viability is a key metric in determining the activity of yeast cells in solution using fluorescent stains like FDA and PI with both cell counters.
Despite the variety of cell counters available, they can typically be divided into one of two sub-groups: manual and automated cell counters.
DeNovix SmartPath™ technology ensures that the measurement distance (pathlength) stays calibrated for the life of the instrument and enables micro-level pathlength control. This results in accurate, reproducible results without the need for routine recalibration.
The Trypan Blue app on CellDrop™ Series instruments enables rapid automated cell counting and viability of cell suspensions stained with Trypan Blue.