DeNovix Instrumentation, Technology and Developments

20 Dec

Fluorescence Assays and the Benefits of Multiple Fluorescence Channels

Fluorescence assays are critical in biochemical and life sciences applications using fluorometry for sample quantification. These comprise fluorophores that bind specifically to analytes of interest and exhibit distinct excitation and emission profiles. In principle, the intensity of light emitted is directly correlative to sample concentration. Leading-edge fluorescence assays enable extremely precise biomolecule detection with, for

Read More
26 Nov

Fluorescence Analysis: 4 Differences Between Absorbance and Fluorescence

Absorbance and fluorescence analysis are distinct yet complementary techniques used to detect and quantify analytes of interest in a sample. Measuring the absorbance of ultraviolet and visible (UV-Vis) light at specific wavelengths is one of the most established methods of quantitating biomolecules. Fluorescence analysis, meanwhile, has long been used to measure the emission spectra of

Read More
02 Nov

Microvolume Analysis of Protein Samples

Measuring the UV-light absorption of proteins is the most straightforward method of microvolume protein analysis for concentration and purity. The aromatic amino acids and residues of many proteins exhibit strong ultraviolet absorption with a peak at 280nm.  The amount of light absorbed is proportional to the concentration of the sample. Using the Beer-Lambert equation, it

Read More
16 Oct

What is a UV-Vis Spectrophotometer?

Ultraviolet-visible (UV-Vis) spectrophotometry is a technique used to measure light absorbance across the ultraviolet and visible ranges of the electromagnetic spectrum. When incident light strikes matter it can either be absorbed, reflected, or transmitted. The absorbance of radiation in the UV-Vis range causes atomic excitation, which refers to the transition of molecules from a low-energy

Read More
21 Sep

The Working Principle of a Fluorometer

Fluorometers are used for precise quantitation of biological molecules including nucleic acids and proteins in microliter (μl) samples. They can measure the concentration of double- and single-stranded DNA (dsDNA and ssDNA), ribonucleic acid (RNA), and proteins in solution with demonstrable applications in a range of biochemical fields. Life science, environmental monitoring, pharmaceutical quality control, and

Read More