Technical Note 172

DeNovix DS-11 Series Fluorometer vs. Thermo Fisher Qubit™ Fluorometer Comparison

Introduction

The DeNovix DS-11 Series Spectrophotometer / Fluorometer and the Thermo Fisher Qubit™ 3.0/4 fluorometer are commonly used for fluorescence quantification of nucleic acids, proteins and other biomolecules. This note presents a comparison of instrument features and system performance of the DeNovix DS-11 Series fluorometer and the Thermo Fisher Qubit™ 3.0/4 fluorometer. A performance comparison of DeNovix dsDNA Quantification Assays and Thermo Fisher Qubit™ dsDNA assays is also presented.

The DS-11 Series fluorometer enables precise fluorescence quantification using a proprietary optical core and a versatile set of four user-selectable fluorescence channels. The DS-11 Series fluorometer, combined with DeNovix dsDNA Fluorescence Assays, provides the highest sensitivity and widest dynamic range for quantification available. When compared to the Thermo Fisher Qubit™ 3.0/4 fluorometers and Qubit™ dsDNA assays, the DeNovix DS-11 Series fluorometer provides a detection limit 20X below Qubit™ and an upper range 2X higher.

 

DeNovix dsDNA Quantification Assays

DeNovix offers three dsDNA Fluorescence Quantification Kits that enable quick, sensitive and reproducible measurements of dsDNA, ranging from 0.5 pg/µL to 4000 ng/µl in a simple mix-and-measure protocol. DeNovix Broad Range, High Sensitivity and Ultra High Sensitivity Assays provide significantly enhanced dynamic range over Thermo Fisher Qubit™ assays (Figure 1).

The DeNovix Ultra High Sensitivity dsDNA Assay delivers unmatched sensitivity, measuring concentrations as low as 0.5 pg/µL. Thermo Fisher’s Qubit™ has no equivalent assay.

Due to DeNovix’s 20X greater sensitivity over the Thermo Fisher Qubit™ HS dsDNA assays, scientists are better equipped for research than ever. Single cell analysis, laser captured samples, circulating DNA and tumor heterogeneity studies are among the applications that can now benefit from faster and more accurate quantification.

Figure 1: Comparison of dsDNA Fluorescence Assay Concentration Ranges

Feature Comparison

The DeNovix DS-11 Series fluorometer includes features equivalent or superior to Thermo Fisher’s Qubit™ 3.0/4 fluorometers. Table 1 summarizes each system. DeNovix fluorometers are compatible with DeNovix Assays and a wide range of other fluorescence assays. Intuitive EasyApps™ software comes preinstalled on this stand-alone instrument. The system includes a custom operating system and a 7” HD touchscreen.

Table 1: Feature Comparison of the DeNovix DS-11 Series and Thermo Fisher Qubit™ 3.0 / 4.0 Fluorometers
FeatureDeNovix
DS-11 Series Spectrophotometer / Fluorometer
Thermo Fisher
Qubit™ 3.0/4 Fluorometers
Fluorescence Channels4 (UV, Blue, Green, Red)2 (Blue, Red)
dsDNA Assay Concentration Range0.5 pg/μL – 4000 ng/μL*10 pg/μL – 2000 ng/μL**
Minimum Sample Volume1 μL (in 200 μL assay volume)1 μL (in 200 μL assay volume)
Measurement Time2 seconds5 seconds
Standard Curves Options2-point standard or 
customizable 2 – 8 standards2 or 3 point standard
Compatible AssaysWide range of assays including commonly used DeNovix, Thermo Fisher Qubit™, and Promega™ AssaysThermo Fisher Qubit™ Assays
UV AssaysPossible including: Alexa Fluor 405, Hoechst 33258, DAPI, eBFP, 7-hydroxy 4- methylcoumarin and 4-Methylumbelliferyl B-D-Galactopyranoside None
Display7” HD Touchscreen5.7” Touchscreen
Password Protected AccountsYesNot Available
Method DevelopmentEasy method developmentRequires use of online tool & subsequent import
Accessory SupportUSB keyboard, mouse, 
barcode readerNot Available
Data ExportWiFi/ethernet to network drives, Email results, USB; LIMS compatibleUSB
NetworkingWiFi and EthernetNot Available
PrintingWiFi printing or local label printerNot Available
Onboard Sample Storage Capacity32 GB storage, >8 million samples4 GB storage, 1000 samples
Software UpdatesWiFi, ethernet, USB; 
Automatic notificationsUSB
ColorsArctic White, Brazilian Blue,
Tungsten Silver, Fire Red
White
Warranty2 years1 year
Country of ManufactureUSAMalaysia

*   Using DeNovix dsDNA Assays
** Using Thermo Fisher Qubit™
dsDNA assays

Methods

Comparison data for DeNovix dsDNA Quantification Assays and correlating Thermo Fisher Qubit™ quantification assays were obtained on a DeNovix DS-11 Series fluorometer and Thermo Fisher Qubit™ 3.0 fluorometer respectively. Each assay was prepared as described in the manufacturer’s protocol. Samples were mixed and incubated at room temperature for five minutes. Three replicate measurements were taken for each sample. The DeNovix Assays were measured on the DeNovix fluorometer, and the Thermo Fisher Qubit™ assays were measured on the Qubit™ 3.0 fluorometer.

DeNovix Broad Range, High Sensitivity and Ultra High Sensitivity Assays

For Broad Range and High Sensitivity, a series of dilutions of calf thymus DNA was prepared in TE buffer. Working solution (190 µL) was added to a thin-walled, clear UV-transparent 0.5 mL PCR tube (DeNovix cat #TUBE-PCR-0.5-500). dsDNA (10 µL) was added to each tube in the standard range, and volume was adjusted for total mass in the extended range.

For Ultra High Sensitivity, working solution (200 µL) was added to a thin-walled, clear UV-transparent 0.5 mL PCR tube. dsDNA (10 µL) was added to each tube.

Thermo Fisher Qubit™ Broad Range and High Sensitivity Assays

A series of dilutions from phage lambda DNA was prepared in TE buffer. Working solution (190 µL) was added to a thin-walled, clear UV-transparent 0.5 mL PCR tube. dsDNA (10 µL) was added to each standard tube, and volume was adjusted for total mass within the core range of the assay. The extended range of the Thermo Fisher Qubit™ assays increases the total mass limitations of the assay. The appropriate total mass for the extended range was added to each assay tube using 200 µL total volumes.

Performance Data

Table 2: Performance Comparison of DeNovix Broad Range Assay Measured on a DeNovix DS-11 FX+ and Thermo Fisher Qubit™ Broad Range Assay Measured on Qubit™ 3.0 / 4.0 Fluorometers
Broad Range dsDNA Assay
ExpectedDeNovix Assay measured on DS-11 FXThermo Fisher Qubit™ Assay measured on Qubit™ Fluorometers
ng/µLng/µL%CVng/µL%CV
40003551.610.010Out of Assay Range
30003123.240.008Out of Assay Range
20001974.530.0071826.333.340
10001050.920.007896.671.897
400358.080.073481.332.288
200195.700.140196.671.471
100106.100.08990.532.053
5053.750.09545.202.341
2526.830.11221.001.905
12.513.480.12612.371.683
6.256.650.3015.912.493
22.390.4602.040.980
11.121.3390.8102.506
0.50.5420.0000.6251.293
0.20.2374.6410.3456.083
0.10.1164.310Measured Out of Range
Table 3: Performance Comparison of DeNovix High Sensitivity Assay Measured on DeNovix DS-11 FX+ and Thermo Fisher Qubit™ High Sensitivity Assay Measured on Qubit™ 3.0 / 4.0 Fluorometers
High Sensitivity dsDNA Assay
ExpectedDeNovix Assay measured on DS-11 FXThermo Fisher Qubit™ Assay measured on Qubit™ Fluorometers
ng/µLng/µL%CVng/µL%CV
250261.620.006Out of Assay Range
10098.070.027112.671.025
2524.780.01626.331.096
1010.570.36010.131.140
33.040.1973.172.026
10.7900.1011.4760.889
0.30.2450.1630.7030.164
0.10.0700.0000.1191.788
0.030.0190.0000.0721.520
0.010.0052.0000.0192.859
0.0050.0045.0000.0084.619
Table 4: Performance Comparison of DeNovix Ultra High Sensitivity Assay Measured on DeNovix DS-11 FX+ and Thermo Fisher Qubit™ Fluorometer
Ultra High Sensitivity dsDNA Assay
ExpectedDeNovix Assay measured on DS-11 FXThermo Fisher Qubit™ Assay measured on Qubit™ Fluorometer
pg/µLpg/µL%CVpg/µLStDev
300304.400.108No Equivalent Assay
150142.910.182
5045.180.398
108.5191.056
21.8322.238
11.0858.750
0.50.3606.890

Summary

The combination of the DeNovix DS-11 Series fluorometer and DeNovix dsDNA Quantification Assays provides fast and accurate measurements with unmatched sensitivity. The DS-11 Series fluorometer and DeNovix Assays enable superior accuracy, higher sensitivity and a broader range of dsDNA quantification (0.5 pg/µL – 4000 ng/µL) compared to the Thermo Fisher Qubit™ 3.0/4 fluorometers and Qubit™ assay range (10 pg/µL – 2000 ng/µL).

Fluorometer mode is available on the DS-11 FX and DS-11 FX+ Spectrophotometer / Fluorometer and the QFX Fluorometer. DeNovix dsDNA Quantification Assays are available for purchase on our webstore and through DeNovix authorized distributors.

Qubit™ is a registered trademark of Thermo Fisher Scientific and its subsidiaries and is used for identification and references purposes only. DeNovix, DeNovix products and this website are not endorsed or authorized by or in any way affiliated with Thermo Fisher Scientific.

Promega™ is a registered trademark of Promega™ Corporation and its subsidiaries and is used for identification and references purposes only. DeNovix, DeNovix products and this website are not endorsed or authorized by or in any way affiliated with Promega™ Corporation.

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8-NOV-2023