Three assay sizes are available. The volume of components in each kit are sufficient for 1000, 250 and 50 (evaluation size) assays respectively. Kit components are shown in the table below.
|DeNovix dsDNA High Sensitivity Dye (100x)||2 x 1 mL||0.5 mL||100 µL|
|DeNovix dsDNA High Sensitivity Buffer||200 mL||50 mL||10 mL|
|25 ng/µL dsDNA Standard (calf thymus)||2 mL||1 mL||0.5 mL|
|0 ng/µL dsDNA Standard||2 mL||1 mL||0.5 mL|
Dynamic Range Considerations
The sensitivity of the Qubit® 3.0 and Qubit® 4 Fluorometers decrease the dynamic range of DeNovix High Sensitivity Assay. For this reason, the 25 ng/µL standard must be diluted to 15 ng/µL when using the Qubit 3.0 or Qubit® 4. The assay is linear on the Qubit® 3.0 / 4 as high as 150 ng/µL when adjusting sample volumes between 1 and 20 µL.
Installing DeNovix Assays on a Qubit® Fluorometer
For a Qubit® 3.0 / 4 Fluorometer:
Once the assay file is uploaded to the Qubit® Fluorometer, simply select the DeNovix High Sensitivity Assay to measure dsDNA samples.
Three replicates of dsDNA samples were measured on a Qubit® 3.0, a Qubit® 4 and a DeNovix QFX Fluorometer using the DeNovix High Sensitivity Assay. Each sample was prepared by adding 10 µL stock to 190 µL working solution. The data is presented in graphical and tabular form across the assay dynamic range below.
|Expected dsDNA||Qubit® 3.0||Qubit® 4||QFX|
The DeNovix dsDNA High Sensitivity Assay enables specific, highly sensitive dsDNA quantitation across a wide dynamic range. The assay is available for use on a Qubit® Fluorometer through the simple application of downloadable assay files.
Qubit® is a registered trademark of Thermo Fisher Scientific and its subsidiaries.
Revised 19 Oct 2020