Kit Contents
Kits include a solution of 0.02% Erythrosin B. The dye should be stored at 4 degrees Celsius in a light-protected tube.
| Assay Size | Erythrosin B Original Concentration | Number of Tests |
|---|---|---|
| 0.25 mL | 0.02% | 50 |
| 1.5 mL | 0.02% | 300 |
Sample Volume and Chamber Height
The required sample volume for the CellDrop depends on the height of the measurement chamber, which is set in the counting protocol.
Standard Magnification (FLi & BF)
| Gap Height (um) | Volume (uL) | Minimum Density (cells/mL) | Maximum Density (cells/mL) |
|---|---|---|---|
| 400 | 40 | 7.0E+02 | 3.1E+06 |
| 100 | 10 | 2.9E+03 | 1.3E+07 |
| 50 | 5 | 5.9E+03 | 2.5E+07 |
| Gap Height (um) | Volume (uL) | Minimum Density (cells/mL) | Maximum Density (cells/mL) |
|---|---|---|---|
| 400 | 40 | 4.3E+03 | 2.6E+07 |
| 100 | 10 | 1.7E+04 | 1.0E+08 |
| 50 | 5 | 3.4E+04 | 2.1E+08 |
Best Practices
- Ensure that the upper and lower chamber surfaces are clean prior to loading sample.
- Lower the arm prior to dispensing sample into the measurement chamber.
- Mix cells immediately before loading sample and avoid introducing air bubbles.
- Follow the image guides to adjust focus and exposure.
- Allow cells to settle and stop moving across the live preview before pressing the Count button.
Sample Prep
- Vortex cell suspension prior to use.
- Mix Erythrosin B and cell suspension together at the desired ratio and mix well. There is no incubation time required. Refer to this table:
| Erythrosin B Volume | Cell Volume | Protocol Dilution Factor | Recommended Exposure |
|---|---|---|---|
| 5 µL 0.02% | 5 µL | 2 | Normal |
Sample Measurement
- With the CellDrop arm in the down position, launch one of the Erythrosin B apps.
- Set sample name, information and protocol as appropriate. If mixing cells and Erythrosin B in a ratio other than 1:1, edit the Dilution Factor in the protocol.
- Pipette well-mixed cells + Erythrosin B solution and dispense appropriate sample volume into the measurement chamber, using the groove on the lower sample surface as a pipetting guide.
- Note: The volume of sample required depends on the protocol settings for the chamber height. The required volume is displayed on the Count button.
- Adjust exposure and focus according to the image guide.
- Allow cells to settle, then press the Count button.
Refer to Technical Note 186 – CellDrop Best Practices for additional guidance and Technical Note 226 for a comparison of Erythrosin B with AO/PI and Trypan Blue.
Refer to denovix.com/sds for safety data sheets for CellDrop Cell Counting Assays.
25-SEP-2024
