The use of trypan blue can be problematic for counting cells and assessing viability in some sample types where debris or non-nucleated cells can interfere. The DeNovix Acridine Orange (Ao) / Propidium Iodide (PI) fluorescence assay is a simple, accurate alternative. Comparative data is presented here.
The NIST has proposed a robust method to assess cell counting linearity and reproducibility. This technical note presents data for the CellDrop using this method and compares results to manually cell counting.
GFP is a common marker for transfection in biological systems. In order to assess transfection success it is important to optimize cell counting settings. this technical note provides a detailed protocol for optimizing the CellDrop GFP Count App.
CellDrop Automated Cell Counters use a patented DirectPipetteTM Technology to replace plastic slides. This method allows variable chamber heights to enhance the cell density range without the need for diluting or concentrating samples.
EasyApps software allows for quick and effective storage, processing and sharing of data. The various options available are presented in this short technical note.
This technical note outlines seven top tips from the DeNovix applications team to ensure accurate and reproducible results when counting cells manually or using automated systems such as the CellDrop™ Automated Cell Counter.