The choice of measurement method for proteins depends on sample type, buffers used and the protein of interest. Colorimetric assays are commonly used for cell lysates and samples in buffers that interfere with direct quantification at 280nm. Common assays and results using microvolume analysis are presented in technical note 108.
Microvolume spectrophotometric measurements are now routine in many life science research labs. As with all microvolume techniques, it is important to apply the best practices to reduce user error and ensure reliable results from a DeNovix® DS-11 Series instrument.
The DeNovix® DS-11 Series Spectrophotometer / Fluorometer is a compact, stand-alone instrument with an intuitive and easy-to-use icon driven software. Separate preconfigured applications for each of the most commonly measured biomolecule types ensure that methods are optimized and simple to use.
To highlight the precision and accuracy of the instrument over a the most commonly used range of the DS-11 Series, a dataset of a dilution series of purified double stranded DNA is presented demonstrating the expected performance with regard to linearity and reproducibility.