The DeNovix® DS-11 Series Peptide app enables protein or peptide concentration determinations based upon absorbance values at either 215 nm or 205 nm. Data is presented for polymyxin B demonstrating the DS-11 can be used to determine the concentration of peptides over a wide dynamic range.
Purity ratios are routinely used to identify contamination in samples when quantifying using UV-Vis. Understanding their utility and threshold values is essential to correct interpretation.
SmartPath Technology enables the DS-11 Series to measure ultra-high concentrations. In this technical note, the relationship between transmittance and absorbance are discussed in the context of the Beer-Lambert Law to explain how the industry-leading dynamic range of the DS-11 is achieved.
Learn about ultraviolet-visible (UV-Vis) spectrophotometry, a technique used to measure light absorbance across the ultraviolet and visible ranges of the electromagnetic spectrum.